Two marker groups in one NGS test
Crohn’s disease and celiac disease are distinct chronic gastrointestinal conditions with partially overlapping clinical features. Both are influenced by genetic and environmental factors, but each is associated with a different set of genetic predisposition markers.
fastGEN Crohn’s & Celiac Disease Kit enables genotyping of ten selected regions:
- Five markers associated with celiac disease: regions associated with the HLA-DQ2.2, HLA-DQ2.5 and HLA-DQ8 haplotypes.
- Five variants associated with Crohn’s disease: selected variants in the IL23R and NOD2 genes.
These markers represent only part of the genetic background of the two multifactorial conditions. The results provide information on genetic predisposition; they are not intended to confirm a diagnosis on their own.
Amplification and indexing in a single PCR
The starting material is isolated DNA. The kit has been validated for DNA isolated from peripheral blood and buccal swabs. Up to 16 samples, including controls, can be processed in a single sequencing run.
fastGEN technology uses short amplicons and tagged hybrid primers to amplify target sequences and index samples in a single PCR. Ready-to-use Master Mixes contain all the necessary reaction components, including indexes. Laboratory staff simply add isolated DNA and perform amplification in a real-time PCR thermal cycler. Combining amplification and indexing reduces sample transfers and manual pipetting steps, lowering the risk of pipetting errors.
Following amplification, the individual libraries are combined into a single pool for purification and quantification. Library preparation takes less than three hours, with under 30 minutes of hands-on time.
From libraries to results
The resulting libraries can be sequenced on any Illumina platform. FASTQ data are then processed by the fastGEN module in GENOVESA, a cloud-based bioinformatics platform.
The automated workflow includes data quality control, alerts for regions with insufficient coverage, variant filtering and visualization, and report generation. Laboratories therefore do not need to develop their own bioinformatics pipeline for this panel.
The entire workflow, from isolated DNA to results, can be completed in approximately 24 hours, depending on the sequencing platform and laboratory workflow.
What sets fastGEN apart?
For laboratories studying this defined set of markers, fastGEN offers a ready-to-use alternative to running separate tests or developing an in-house targeted NGS panel.
New to the fastGEN portfolio
The fastGEN Crohn’s & Celiac Disease Kit expands BioVendor’s offering for gastroenterology and genetics research. Detailed specifications and instructions for use are available on the product page to help you assess whether the kit meets your laboratory’s needs.
Follow two leads with one test.
Discover the fastGEN Crohn’s & Celiac Disease Kit
For research use only.
