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LAMP

Revolution in area testing.  

LAMP (Loop Mediated Isothermal Amplification) is a highly sensitive, reliable, fast and efficient method of nucleic acid amplification and detection. Kits based on the LAMP principle can be applied directly to the primary sample, swab in a suitable medium, and thus reduce handling steps. Results are available within 30-40 minutes.

LAMP

Loop Mediated Isothermal Amplification (LAMP) technology offers a wide range of applications from pathogen detection to single nucleotide polymorphism determination. The target sequence is amplified without thermocycling with using a set of loop primers, a fluorescently labeled probe and an isothermal polymerase which, in addition to replication activity, is the ability to dissociate and shift the strand. The presence of loop primers increases the rate and dynamics of the reaction, and therefore the amount of nucleic acid generated by LAMP is higher than number of nucleic acid copies generated by conventional polymerase chain reaction. This characteristic significantly accelerates the detection of the target sequence.  

Diagnostic kits based on the LAMP principle are designed to be easy to use, with a significant pipetting steps reduction; robust, with the possibility of using a sample (swab) without nucleic acids isolation, fast with results within 30 minutes, accurate and reliable. These properties make LAMP an ideal method for area testing.  

SARS-CoV-2 Direct LAMP 96-kit

The kit was developed for SARS-CoV-2 nucleic direct acid detection. No DNA isolation is required, nasopharyngeal swab in non-inactivating Viral Transport Medium (VTM) is deactivated by the short thermal deactivation. This step is followed by reverse transcription (RT) and amplification based on the LAMP principle. The reaction targets the S gene of viral RNA and can be performed by a wide range of real time PCR cyclers available in any genetic laboratory. The kit is designed as "ready to use" format to perform 96 reactions and contains. All reagents are included in LAMP Master Mix, the kit also include a Positive Control (PC) to check that the reaction is correct, and Nuclease Free Water (NC) to perform a negative control reaction (exclusion of contamination).

SARS-CoV-2 Direct MULTILAMP 96-kit

The SARS-CoV-2 Direct MULTILAMP 96-kit offers the same benefits of use as the SARS-CoV-2 Direct LAMP 96-kit - fast, accurate and sensitive detection of viral RNA from nasopharyngeal swabs. In addition to the S gene, the target sequence of human DNA (RNaseP) is also amplified at the same time as a control for the course of the reaction.  

More about LAMP

Are you interested in more?

Contact our project manager.

Kristýna Brzobohatá, Ph.D.
Project Manager

+420 724 873 015
brzobohata@biovendor-mdx.com

Kristýna Brzobohatá, Ph.D.

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